Coding

Part:BBa_K4482005:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-08-25)


NTRK3 eGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1840
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal PstI site found at 489
    Illegal PstI site found at 742
    Illegal NgoMIV site found at 134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2483
    Illegal SapI site found at 435
    Illegal SapI site found at 1089


Design Notes

The primers used for the PCR were designed to allow for Gibson assembly.


Source

This part was generated by PCR from plasmids obtained from AddGene.

References